LIFE SCIENCES

Siekhaus Group

Invasive Migration

The ability of cells to migrate is crucial for their function in the immune system, the formation of the body, and the spread of cancer. The Siekhaus group investigates how cells move within the complex environment of an organism, using the genetic power of the fruit fly to interrogate this process and identify ways in which it is regulated.

Vertebrate immune and cancer cells need to squeeze between closely connected cells to disseminate in the body. Daria Siekhaus and her group study how cells penetrate such tissue barriers, using the developmental movement of macrophages in the fruit fly Drosophila melanogaster as a model. The Siekhaus group uses a combination of imaging, genetics, cell biology, biophysics and modeling to identify the strategies that underlie tissue invasion. The group has found that a cytokine conserved in vertebrates facilitates tissue entry by reducing tension in surrounding tissues. The group has defined a novel program acting in disseminating and invading macrophages that alters glycosylation to aid invasion; this program displays molecular conservation in metastatic cancer cells. They have also defined a conserved pioneer invader program acting in macrophages that activates transcription and translation of an mRNA subset to increase tissue entry.

Group Leader


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Team


Current Projects

The role of cell division in regulating invasive migration | A novel transporter and its effect on glycosylation, immune function and metastasis | A conserved pioneer cell program that governs invasion through transcriptional and translational shifts


Publications

Valosková K, Biebl J, Roblek M, Emtenani S, György A, Misova M, Ratheesh A, Rodrigues P, Shkarina K, Larsen ISB, Vakhrushev SY, Clausen H, Siekhaus DE. 2019. A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion. eLife. 8, e41801. View

Ratheesh A, Biebl J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W, György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell. 45(3), 331–346. View

György A, Roblek M, Ratheesh A, Valosková K, Belyaeva V, Wachner S, Matsubayashi Y, Sanchez Sanchez B, Stramer B, Siekhaus DE. 2018. Tools allowing independent visualization and genetic manipulation of Drosophila melanogaster macrophages and surrounding tissues. G3: Genes, Genomes, Genetics. 8(3), 845–857. View

Matsubayashi Y, Louani A, Dragu A, Sanchez Sanchez B, Serna Morales E, Yolland L, György A, Vizcay G, Fleck R, Heddleston J, Chew T, Siekhaus DE, Stramer B. 2017. A moving source of matrix components is essential for De Novo basement membrane formation. Current Biology. 27(22), 3526–3534e.4. View

Ratheesh A, Belyaeva V, Siekhaus DE. 2015. Drosophila immune cell migration and adhesion during embryonic development and larval immune responses. Current Opinion in Cell Biology. 36(10), 71–79. View

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Career

since 2012 Assistant Professor, IST Austria
2003 – 2011 Research Scientist, Skirball Institute, New York University Medical Center, USA
1999 – 2003 Postdoctoral Fellow, University of California, Berkeley, USA
1998 PhD, Stanford University, USA


Selected Distinctions

2019 Chosen for “Excellence in Peer Review” by Developmental Cell editors
2018, 2019 F1000 Prime highlighted papers
2016 FWF Grant
2012 Marie Curie Career Integration Grant
2003 – 2005 NIH Fellowship


Additional Information

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