Skip to main content
Home
Research
Scientific Service Units
Imaging & Optics Facility
Microscopy

Microscopy

2 x Inverted widefield microscope Nikon Eclipse / Ti-2E

  • Widefield fluorescent microscope
  • Lumencor SpectraX light source
  • Motorised stage
  • cameras:
    • Nikon Eclipse: color (Nikon DS-FI) and monochrom (Hamamatsu EMCCD 9100)
    • Nikon Ti-2E: DS-Qi2
  • Temperature control chamber
  • external phase contrast and DIC  imaging optics
  • 1 system (Nikon-Ti-2E) with automated experiment design option

Upright LSM 800 confocal microscope

  • Laser lines 405, 488, 555, 640 nm
  • 2 GaAsP PMT’s for reflected light detection
  • PMT for transmitted light detection
  • Multiple position imaging + automated experiment design option
  • Temperature chamber available

3 x Inverted LSM 800 confocal microscope

  • 3 x Laser lines 405, 488, 555, 640 nm
  • 2 setups
    • 1 x MA PMT’s for reflected light detection
    • 2 x GaAsP PMT’s for reflected light detection
  • ESID + LED electronically switchable illumination and detection module 
  • Multiple position imaging + automated experiment design option
  • The 2 systems with GaAsP detectors are equipped with incubation chamber and CO2/N2 mixer

2 x Vertical LSM 800 confocal microscope

  • Microscope is turned at 90° to enable observation of sample in a proper position to gravity
  • Laser lines: 405, 488, 555, 640 nm
  • 2 MA-PMT’s for reflected light detection
  • ESID + LED electronically switchable illumination and detection module 
  • Multiple position imaging  + automated experiment design option
  • Rotation stage to change the angle of a sample in relation to gravity
  • Custom-programmed ‘root tracker’ software to adjust stage position to the sample movement

Upright LSM 880 confocal microscope with Airyscan

  • Laser lines 405, 458, 488, 514, 561, 640 nm
  • 2 PMT’s and 1 GaAsp (single channel) detector for reflected light detection
  • Airyscan detector
  • PMT for transmitted light detection
  • Multiple position imaging  + automated experiment design option
  • Equipped with incubation chamber for temperature and a gas-mixer

Inverted LSM 880 confocal microscope with Airyscan

  • Laser lines 405, 458, 488, 514, 561, 640 nm 
  • 2 PMTs  and 1 GaAsp (single channel) detector for reflected light detection
  • Airyscan detector
  • PMT for transmitted light detection
  • Multiple position imaging  + automated experiment design option
  • Equipped with incubation chamber for temperature and a gas-mixer

Upright SP5 confocal microscope

  • Laser lines 405, 458, 488, 514, 561, 633
  • 5 PMTs with free selection of detection range
  • TL detector for transmitted light
  • Motorized stage for multiple position imaging
  • Temperature-control chamber

Inverted SP5 confocal microscope

  • Laser lines 458, 488, 514, 561, 633 
  • 3 PMTs  and 2 hybrid detectors (Leica HyD) with free selection of detection range
  • transmitted light detector
  • Tandem scanner for ultrafast imaging
  • Heated stage and objective heater
  • FRET and FRAP software
  • Micromanipulators / microfluidic pump

Upright multiphoton microscope and FLIM Detector

  • Upright 2-photon microscope
  • Multiphoton imaging with two laser lines (Cameleon Ultra II  and OPO compact), wavelength range  780-1300 nm  
  • Multichannel MP imaging with up to 4 NDD (all GaAsp detectors) simultaneously
  • 2 PMTs in a transmitted port, for SHG/THG imaging
  • Incubation chamber for temperature control
  • Can be equipped with portable PMT based FLIM X16 TCSPC [Time Correlated Single Photon Counting] detector 

Inverted multiphoton microscope

  • Inverted 2-photon microscope
  • Multiphoton imaging with two laser lines (Cameleon Ultra II  and OPO compact), wavelength range  780-1300 nm  
  • Multichannel MP imaging with up to 3 NDD (all GaAsp detectors) simultaneously
  • EOM control of laser power, ROI imaging, linescan
  • 1 PMTs in a transmitted port, for SHG/THG imaging
  • Tabletop sample incubator and portable CO2 mixer
  • Portable PMT based FLIM X16 TCSPC [Time Correlated Single Photon Counting] detector 

Spinning disk & laser cutter microscope

  • Inverted Microscope
  • Spinning Disk confocal CSU-X1
  • 488,561,640 nm laser lines
  • Camera: iXon 897
  • FRAPPA unit
  • Scanning laser microdissection with pulsed 355 nm laser, simultaneous imaging and microdissection possible
  • Motorized piezo stage
  • stage and objective heating unit 
  • Micromanipulators / microfluidic pump

Spinning disc and TIRF microscope iMic

  • Inverted Spinning Disc / multipoint TIRF / epifluorescence microscope
  • Spinning disc confocal unit: Andromeda
  • Spinning disc unit camera: Andor Ixon 885
  • Laser lines: 405, 445, 488, 561, 640 nm
  • Oligochrome light source for epifluorescence imaging
  • Multipoint 360° TIRF (Total Internal reflection)-microscopy with all laser lines, motorized adjustment of TIRF angle calibration 
  • FRAP with all laser lines
  • TIRF/Epifluorescence port is equipped with image splitter (Andor Tucam) with two Ixon 897 cameras
  • Motorized stage for multiposition imaging
  • Temperature control of a sample with tabletop incubator (incubation chamber coming soon)

TIRF / FRAP microscope

  • Automated TIRF/Epifluorescence microscope
  • Multipoint 360° TIRF (Total Internal reflection)-microscopy with all laser lines (iLAS2), motorized adjustment of TIRF angle calibration 
  • Laser lines 405, 488, 561, 640nm
  • Lumencor SpectraX engine for epifluorescence imaging
  • Equipped with FRAP module for simultaneous and sequential bleaching (405 nm)
  • Equipped with climatised chamber- temperature control, CO2 supply
  • DIC imaging is possible combined with epifluorescence

OpenSPIM Light Sheet microscope

  • 488 nm and 561 nm lasers
  • 525/50 BP filter for GFP imaging, dual bandpass filter for green/red imaging 
  • Camera: Hamamatsu Orca R2
  • Temperature controlled sample chamber, peristaltic pump 
  • multiple sample chambers for different RI / sample embedding media
  • OpenSPIM open source platform information website: openspim.org

 

CellHesion Atomic Force microscope

Probing Atomic Force Microscope with possibility of simultaneous fluorescence imaging

  • 2 different scan heads:
    • JPK Cellhesion 200: force spectroscopy
    • Nanowizard 4: AFM scanning head for contact, tipping or quantitative imaging
  • Petri dish heater unit
  • High sensitivity cooled Andor camera
  • in-house developed top-view optics

VS120 Slide Scanner, Olympus

  • Widefield microscope
  • Grayscale and colour camera
  • Light source: X-Cite Excite lamp
  • Filter combinations for DAPI, FITZ, CY3, CY5
  • Motorized stage with 5 slide positions

Inverted widefield Olympus IX83 microscope

  • Widefield fluorescent microscope
  • Lumencor SpectraX light source
  • Motorised stage
  • Hamamatsu  Orca Flash 4.0 sCMOS camera
  • Temperature control chamber


theme sidebar-arrow-up
Back to Top