4. Jun 2019

Understanding Ras Sensitivity to Mutation by High-Throughput Mutagenesis

Datum: 4. June 2019 | 14:00 – 15:30
Sprecher: John Kuriyan, Howard Hughes Medical Institute Departments of Molecular & Cell Biology and Department of Chemistry, UC Berkely
Veranstaltungsort: Big Seminar room Ground floor / Office Bldg West (I21.EG.101)

Ras and many other proteins that are oncogenic, i.e., associated with cancer when mutated, are allosteric switches. The growing power of gene synthesis and next-generation sequencing can be harnessed to yield insights into molecular mechanism in a novel way. I will cover the application of high-throughput mutagenesis to Ras, combined with structural analysis and molecular dynamics simulations, with an emphasis on understanding the allosteric mechanisms that lead to mutational sensitivity.